The Kemmeren group is looking for an enthusiastic, highly motivated research technician who excels in a stimulating, multi-disciplinary research environment
About the Kemmeren group
The Kemmeren group exploits cancer genomics data through a combination of bioinformatics and systems biology approaches with the goal to improve our understanding of childhood cancer initiation and progress as well as improve patient care for children with cancer. Examples include driving bioinformatics research for precision oncology and diagnostics, improving structural variation detection and investigating biological mechanisms of genetic interactions in childhood cancer. We have developed a unique combination of expertise in bioinformatics, functional genomics and molecular-genetic interactions. While our focus is on computational aspects, we collaborate intensively with (pediatric) tumor biology groups, thus enabling a truly multidisciplinary approach. Within the group, we currently have an opening for a research technician within the larger framework of unravelling genetic interactions in pediatric cancer.
Cancers arise and progress through the acquirement of combinations of mutations. Genetic interactions are specific combinations of mutations that have unpredictable phenotypic consequences. Thorough understanding of genetic interactions, their underlying mechanisms and relation to pediatric cancer is crucial for deciphering cancer predisposition, onset and progression as well as for developing precision medicine approaches. Two genetic interaction types are of particular importance for cancer. Detailed understanding of cooperative interactions will aid in elucidating general principles governing cancer onset and progression. Understanding mutually exclusive interactions is pivotal for developing more effective cancer drugs. A detailed molecular understanding of cooperative and mutually exclusive alterations is vital for elucidating general principles underlying these types of genetic interactions in the context of pediatric cancers. Using in vitro cell models (such as cell lines and patient-derived tumor organoids), we will further investigate several of the cooperative and mutually exclusive interactions found. A variety of cell models and experimental procedures will be used, depending on the cancer type and particular genetic interaction(s) involved. This will be performed in close collaboration with the Drost group (organoids and kidney tumors). One example includes a mutually exclusive interaction found between DROSHA and TP53 in Wilms’ tumors for which experimental validation in organoid cultures has already been initiated and which will be further explored in this project.
Tasks and responsibilities
We are looking for a highly motivated and enthusiastic research technician, who excels in a stimulating, multi-disciplinary research environment. The candidate will coordinate and perform experimental follow-up of the predictions derived from the computational work and will be working in both the Kemmeren and Drost groups. Experimental work will involve cell culturing, gene editing in cell lines and organoid cultures using (amongst others) CRISPR-Cas9 technology to validate the identified genetic interactions, as well as a wide variety of molecular biology techniques. Upon validation of the interaction, further experiments will be aimed at addressing the exact mechanism of the interaction involved, for instance using (single cell) mRNA expression analysis to elucidate the processes and pathways involved.
Education and skills
We expect a highly motivated candidate with a bachelor (HBO) in molecular biology or equivalent training. Excellent lab skills and experience with cell culturing, molecular biology techniques, and next generation sequencing techniques are required. Affinity with bioinformatics data analyses is also preferred. The candidate should also have good communication skills, be flexible, versatile and a good team player.
A full time position (36 hours a week) initially for one year, with the intention to extend
for a total period of three years as a research technician. You will be working in a very stimulating and cross-disciplinary research environment, combining cutting-edge research with state-of-the-art molecular technologies. Your gross monthly salary will depend on experience and background, starting in scale 45 with 8,33% gross monthly salary holiday allowance and 8,33 % end-of-year bonus. The Princess Máxima Center operates according to the collective labor agreement ‘cao algemene ziekenhuizen’.
Contact and application
You can apply by pressing the ‘Apply’ button on this screen
Application deadline: Januari 10th 2022.
For more information contact Dr. Patrick Kemmeren (email@example.com).
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