The Kemmeren group is looking for an enthusiastic, highly motivated technician who excels in a stimulating, multi-disciplinary research environment
About the Kemmeren group
The Kemmeren group uses bioinformatics and systems biology to understand the molecular mechanisms underlying genetic interactions in relation to cellular processes and complex human diseases. We have developed a unique combination of expertise in bioinformatics, functional genomics and molecular-genetic interactions. While our focus is on computational aspects, we collaborate intensively with (pediatric) tumor biology groups, thus enabling a truly multidisciplinary approach. Within the group, we currently have an opening for a research technician within a larger KWF funded project dedicated to unravelling genetic interactions in pediatric cancer.
Cancers arise and progress through the acquirement of combinations of mutations. Genetic interactions are specific combinations of mutations that have unpredictable phenotypic consequences. Thorough understanding of genetic interactions, their underlying mechanisms and relation to pediatric cancer is crucial for deciphering cancer predisposition, onset and progression as well as for developing precision medicine approaches. Two genetic interaction types are of particular importance for cancer. Detailed understanding of cooperative interactions will aid in elucidating general principles governing cancer onset and progression. Understanding mutually exclusive interactions is pivotal for developing more effective cancer drugs. A detailed molecular understanding of cooperative and mutually exclusive alterations is vital for elucidating general principles underlying these types of genetic interactions in the context of pediatric cancers. Using cellular models (cell lines and patient-derived stem cell organoid cultures), we will further investigate several of the cooperative and mutually exclusive interactions found. The exact nature of the cellular models and experimental procedures depends on the cancer type and particular genetic interactions involved and will be performed in close collaboration with research groups in the Princess Maxima Center.
The Princess Máxima Center for Pediatric Oncology is a research hospital concentrating healthcare, research and education with regard to cancer in children in a single location in Utrecht. The institute aims to provide the highest level of care for all children with cancer and has the ambition to cure all children of cancer and significantly reduce unwanted side effects. The center brings together the best possible care and scientific research, creating a unique interdisciplinary institute for pediatric oncology in Europe.
Tasks and responsibilities
We are looking for a highly motivated and enthusiastic research technician, who excels in a stimulating, multi-disciplinary research environment. The candidate will coordinate and perform experimental follow-up of the predictions derived from the computational work in close collaboration with and support from the research groups of Frank Holstege (genomics, scRNA-seq), Jarno Drost (Wilms tumors), Esther Hulleman (brain tumors) and Jules Meijerink (T-ALL). Experiments will involve gene editing in cell lines and organoid cultures using CRISPR-Cas9 technology to validate the identified genetic interactions. Upon validation of the interaction, further experiments will be aimed at addressing the exact mechanism of the interaction involved, for instance using mRNA expression analysis to elucidate the processes and pathways involved.
Education and skills
We expect a highly motivated candidate with a bachelor (HBO) in molecular biology or equivalent training. Excellent lab skills and experience with either CRISPR-Cas9, organoid culturing and next generation sequencing techniques are required. Affinity with bioinformatics data analyses are also preferred. The candidate should also have good communication skills, be flexible, versatile and a good team player.
A full time position (36 hours a week) initially for one year, with the intention to extend.
for three years) as a research technician. You will be working in a very stimulating and cross-disciplinary research environment, combining cutting-edge research with state-of-the-art molecular technologies. Your gross monthly salary will depend on experience and background, starting in scale 45 with 8,33% gross monthly salary holiday allowance and 8,33 % end-of-year bonus. The Princess Máxima Center operates according to the collective labour agreement ‘cao algemene ziekenhuizen’.
Contact and application
You can apply by pressing the ‘Apply’ button on the right of this screen
Application deadline: March 31st 2019
For more information contact Dr. Patrick Kemmeren (firstname.lastname@example.org).
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