The Princess Máxima Centre for Pediatric Oncology is a new research hospital concentrating healthcare, research and education with regard to cancer in children in a single location. The institute aims to provide the highest level of care for children with cancer and has the ambition to cure all children of cancer and significantly reduce unwanted side effects. The center brings together the best possible care and scientific research, creating a unique interdisciplinary institute for pediatric oncology in Europe.
The Research Group
The research group led by Olaf Heidenreich is focusing on fusion genes as drivers of childhood leukaemia and targets for novel leukaemia-specific therapies. We are pursuing here a two-pronged approach with, on the one hand, developing drug delivery approaches for the direct inhibition of fusion gene expression, and, on the other, exploring the fusion gene-driven transcriptional networks for crucial “transmitters” that are amenable to pharmacologic intervention. In particular the second part includes the application of targeted and genome-wide RNAi and CRISPR screens. Extending these investigations from cell lines to primary patient material requires complex tissue culture in conjunction with advanced immunodeficient mouse models for xenotransplantation. We therefore develop novel co-culture systems for ex vivo patient cell propagation and harness our mouse models for in vivo validation experiments. The ultimate aim of this research is the design of treatments for childhood leukaemia that are more specific, more effective and less toxic than current chemotherapy and transplantation-based regimens to improve both cure of children with leukaemia and their long-term quality of life.
Tasks and Responsibilities
We are seeking a highly motivated, flexible and energetic research assistant to join the Heidenreich lab. The successful applicant will be an integral part of the lab’s research activities, and will design and conduct a variety of ex vivo and in vivo studies with an emphasis on co-culture systems for leukaemic cell propagation and manipulation. While research will be the major part of the job, the candidate will also operate as a lab manager, which entails requesting and ordering supplies necessary for projects, maintaining frozen stocks of cells and plasmids, and supervising and training new lab members.
Skills and Education:
You are holding BSc or MSc in a relevant biomedical subject. You have considerable knowledge in primary cell culture; expertise in MSC (mesenchymal stroma cells) culture and iPSC (induced pluripotent stem cell) generation and maintenance is a strong plus. Furthermore, you are willing to work with laboratory animals. You are able to analyse data using standard software packages such as Excel or Prism. You will collaborate with other team members and have essential social skills for fruitful/successful interactions. Finally, you are fluent in English and have excellent writing and presentation skills in this language.
Working at the Princess Máxima Center:
We offer an inspiring and interactive research environment, state-of-the-art facilities and possibilities for personal growth and development.
The full time position (36 hours a week) is initially for one year, with the intention to extend.
Your gross monthly salary will depend on experience and background, starting in scale 45 with 8,33% gross monthly salary holiday allowance and 8,33 % end-of-year bonus. The Princess Máxima Center operates according to the collective labour agreement ‘cao algemene ziekenhuizen’.
You can apply by pressing the ‘Apply’ button on the right of this screen
Application deadline: December 19th 2018.
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